Potensi senyawa racemosinin sebagai inhibitor absorbsi kolesterol secara in silico

Dewi Ratih Tirto Sari, Yohanes Bare, Maulidia Riska Pratiwi

Abstract


Kolesterol merupakan molekul yang berperan penting dalam morfogenesis, pembentukan hormon, vitamin, dan mengatur permeabilitas membrane sel di dalam tubuh. Namun, kelebihan kolesterol dapat memicu berbagai macam penyakit seperti obesitas, diabetes mellitus tipe 2, serangan jantung, atherosclerosis, dan stroke. Penelitian ini bertujuan mengidentifikasi potensi racemosin, racemosinin A, dan racemosinin B dalam penghambatan penyerapan kolesterol. Penelitian menggunakan in silico dengan pendekatan docking molekuler. Senyawa racemosin, racemosinin A dan B diinteraksikan dengan protein NPC1L1 dengan kontrol pembanding kolesterol. Docking dilakukan dengan Molegro virtual Docker versi 5 dan divisualisasi dengan Discovery studio versi 21.1.1. Hasil penelitian menunjukkan senyawa racemosin, racemosinin A dan B mengeblok protein NPC1L1 pada daerah sterol – sensing domain yaitu pada residu ALA201, LEU83, dan PHE108. Selain itu, senyawa racemosin juga menghasilkan nilai energi ikatan dalam skor docking yang lebih rendah dari racemosinin A dan B yang mengindikasikan interaksinya dengan NPC1L1 lebih kuat dari kedua senyawa. Pengeblokan ini menyebabkan kolesterol tidak dapat mengikat NPC1L1 dan tidak terserap oleh usus halus. Penelitian ini disimpulkan bahwa senyawa racemosin, racemosinin A dan B berpotensi sebagai inhibitor NPC1L1 dan menghambat penyerapan kolesterol, sehingga kolesterol tubuh mengalami homeostasis.

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