Profil senyawa flavonoid daun jati (Tectona grandis) menggunakan Liquid Chromatography-Mass Spectrometry

Waris Waris, Mohammad Arfi Setiawan, Cicilia Novi Primiani

Abstract


Tumbuhan jati (Tectona grandis) merupajan tumbuhan dengan persebaran luas di Indonesia. Kandungan fitokimia tumbuhan belum banyak dilakukan analisis, sehingga kemanfaatan sebagai tumbuhan obat masih kurang. Tujuan penelitian untuk menganalisis senyawa golongan flavonoid daun jati dengan metode Liquid Chromatography-Mass Spectrometry (LCMS). Purifikasi ekstrak menggunakan C18 Sep-Pak dengan spesifikasi column shimadzu shim pack FC-ODS (2 mm x 150 mm 3µm), Injection volume 1µl, column temper 35ºC, mobile phase mode isocratic, flow digunakan adalah etanol 96%. Hasil analisis LCMS ditemukan adanya senyawa golongan flavonoid yaitu quercetine 1,42081% dan senyawa rutin 1,31488%. Analisis fitokimia sangat penting, sehingga dapat dimanfaatkan sebagai data dalam pengembangan tanaman obat.


Keywords


flavonoid; LCMS; quercetin; rutin; tumbuhan jati

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References


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