Ratih Rinendyaputri, Uly Alfi Nikmah


The cause of infertility can be caused by a disorder of female replacement. The inability to ovulate (anovulation) causes no fertilization is one of the main causes of infertility. Assisted reproductive technology (ART) can be one solution to help replace fertilization. However, in vitro maturation of oocyte (IVM) can lose the success of fertilization. Oocyte maturation can be done outside the body (in vitro) by adding various growth factors (GF). Follow the follicles received with the maturation of the egg. Various GFs such as epidermal growth factor (EGF) and insulin growth factor (IGF) so that the egg maturation process occurs, namely maturation of the nucleus and cytoplasm. The maturation of the nucleus with the cell nucleus has succeeded in achieving metaphase II (MII) while the maturation of the cytoplasm is indicated by structural and biochemical complexity in the cytoplasm. The use of GF can be obtained from the secretion of mesenchymal stem cells (MSC), namely coditioned medium (CM-MSC). Growth factors (GF) excreted by MSC such as EGF and IGF can be used to incrised oocyte IVM through of garnulosa/cumulus cells expantion.

Keywords: in vitro maturation (IVM), oocyte, growth factor, CM-MSC

Full Text:



Brugo-Olmedo S, Chillik C, Kopelman S. Definition and causes of infertility

Reproductive BioMedicine. 2000; 2(1): 41-53.

Picton HM, Harris SE, Muruvi W and Chambers EL. The in vitro growth and maturation of follicles. Reproduction. 2008; 136: 703-15.

Liang C, Su Y, Fan Y, Schatten H, and Sun Q. Mechanisms Regulating Oocyte Meiotic Resumption: Roles of Mitogen-Activated Protein Kinase. Molecular Endocrinology. 2007; 21(9): 2037–55.

Sánchez F and Smitz J. Molecular control of oogenesis. Biochimica et Biophysica Acta. 2012; 1822:1896–912.

Ferreira EM, Vireque AA, Adona PR, Meirelles FV, Ferriani RA, Navarro PAAS. Cytoplasmic maturation of bovine oocytes: Structural and biochemical modifications and acquisition of developmental competence. Theriogenology. 2009; 71:836–48.

Sirotkin AV, Dukesov J, MakarevichAV, Kubez A, Bulla J. Evidence that growth factors IGF-I, IGF-II and EGF can stimulate nuclear maturation of porcine oocytes via intracellular protein kinase A. Reprod. Nutr. Dev. 2000; 40: 559–69.

Shen C, Lie P, Miao T, Yu M Lu Q, Feng T,Li J, Zu T, Liu X and Li H. Conditioned medium from umbilical cord mesenchymal stem cells induces migration and angiogenesis. Molecular Medicine Report. 2015;12: 20-30.

Noverina R , Widowati W, Ayuningtya W, Kurniawan D, Afifah E, Laksmitawati DR, Rinendyaputri R, Rilianawati R, Faried A, Bachtiar I, Wirakusumah FF. Growth factors profile in conditioned medium human adipose tissue-derived mesenchymal stem cells (CM-hATMSCs) . Clinical Nutrition Experimental. 2019; 24:33-44.

Majumdar A and Mangal N. S. Hyperprolactinemia. J Hum Reprod Sci. 2013; 6(3): 168–75.

Santoro N. Update in Hyper- and Hypogonadotropic Amenorrhea. J Clin Endocrinol Metab,. 2011; 96(11):3281–88.

Rosenfield RL and Ehrmann DA. The Pathogenesis of Polycystic Ovary Syndrome (PCOS): The Hypothesis of PCOS as Functional Ovarian Hyperandrogenism Revisi. Endocr Rev. 2016; 37(5): 467–520.

Creux H., Monnier P., Son W., Tulandi T., and Buckett W. Immature oocyte retrieval and in vitro oocyte maturation at different phases of the menstrual cycle in women with cancer who require urgent gonadotoxic treatment. Fertility and Sterility. 2017;107(1);198-205.


  • There are currently no refbacks.