FUNGSIONAL METAGENOMIK: STRATEGI EFEKTIF DALAM EKSPLORASI GEN TARGET GLIKOSIDA HIDROLASE
Abstract
Strategi metagenomik memberikan keuntungan dapat mengekplorasi langsung genom-genom yang belum terjelajahi, terutama dari mikroorganisme yang tidak dapat dibudidayakan atau dikultur dalam kondisi tertentu. Penemuan gen-gen yang berisi klon-klon glikosida hidrolase yang sama sekali tidak identik dengan yang ditemukan pada database publik, telah berhasil dilakukan melalui pendekatan metagenomik. Analisis data terhadap hasil temuan-temuan klon-klon pada pustaka metagenomik yang dihasilkan menunjukkan klon yang diperoleh sebagai rangkaian asam nukleat fungsional baru yang berbeda dari database yang sudah ada.
Kata Kunci: Fungsional metagenomik, eksplorasi gen target, glikosida hidrolase
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