PENGARUH SUHU INKUBASI TERHADAP PERSILANGAN JAMUR TIRAM MERAH MUDA (Pleurotus flabellatus) DENGAN JAMUR TIRAM PUTIH (Pleurotus ostreatus) VARIETAS Grey oyster

Abstract

Pleurotus flabellatus is a type of fungus that has the advantage of a shorter life cycle and has high protein content while Pleurotus ostreatus Grey oyster varieties are hybrid seeds as the result of crossbreeding protoplast fusion between oyster mushrooms with oyster mushrooms and white oyster mushrooms. The purpose of this study is to determine whether the treatment of different incubation temperatures, namely with a temperature range of 8-10 ^° C, 18-20 ^° C, 28-30 ^° C and 38-40 ^° C can affect the speed of fusion mycelium and mycelial growth after the fusion process. This cross uses the mycelium of each oyster mushroom which is then crossed using the mycelium fusion method in one bottle containing the PDA media and incubated at a predetermined temperature range. This study showed that the incubation temperature had an effect on the speed of the occurrence of the wedge connection and the growth of the mycelium after the fusion process. In the Kruskall-wallis test showed that there was an effect of incubation temperature on the fusion velocity by marking the formation of a wedge joint and there was an effect of incubation temperature on the growth of mycelium after the fusion process. Connection susceptible wedge formed at temperatures (18-20, 28-30 and 38-40) ^o C and a temperature range of 28-30 ^° C is the best temperature that can be used as an incubation temperature at the intersection of pink oyster mushrooms (Pleurotus flabellatus) with white oyster mushroom (Pleurotus ostreatus) Grey oyster varieties with the criteria of the wedge connection speed, the thickness of the mycelium and endurance until the optimal limit of mycelium growth