POTENSI GROWTH FACTOR (GF) DARI SEL PUNCA MESENKIM UNTUK PEMATANGAN SEL TELUR SECARA IN VITRO

Ratih Rinendyaputri, Uly Alfi Nikmah

Abstract


The cause of infertility can be caused by a disorder of female replacement. The inability to ovulate (anovulation) causes no fertilization is one of the main causes of infertility. Assisted reproductive technology (ART) can be one solution to help replace fertilization. However, in vitro maturation of oocyte (IVM) can lose the success of fertilization. Oocyte maturation can be done outside the body (in vitro) by adding various growth factors (GF). Follow the follicles received with the maturation of the egg. Various GFs such as epidermal growth factor (EGF) and insulin growth factor (IGF) so that the egg maturation process occurs, namely maturation of the nucleus and cytoplasm. The maturation of the nucleus with the cell nucleus has succeeded in achieving metaphase II (MII) while the maturation of the cytoplasm is indicated by structural and biochemical complexity in the cytoplasm. The use of GF can be obtained from the secretion of mesenchymal stem cells (MSC), namely coditioned medium (CM-MSC). Growth factors (GF) excreted by MSC such as EGF and IGF can be used to incrised oocyte IVM through of garnulosa/cumulus cells expantion.

Keywords: in vitro maturation (IVM), oocyte, growth factor, CM-MSC


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